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A BLAST search identified five proteins with relevant known structures: the NitFhit protein from Caenorhabditis elegans (pdb-code 1EMS) [21]; hypothetical protein Ph0642 from Pyrococcus horikoshii (1J31) [22]; Nit3 protein from Saccharomyces cerevisiae (a member of branch 10 of the nitrilase superfamily, pdb-code 1F89) [23]; the pyrimidine degrading enzyme from Drosophila melanogaster (2VHH) [24] and mouse nitrilase-2 (2W1V) [25] with corresponding identities of 22, 23, 20, 21 and 22%. Although the percentage of identity is at the lower threshold for homology modelling, 3D alignment with the SHEBA plug-in in YASARA showed a high conservation of secondary structure elements among the selected templates, thus supporting the attempt to at least obtain a useful low-resolution homology model. The C-terminal part (residues 316-356) was modelled based on the crystal structure of kinesin from Rattus norvegicus (2KIN, 29% of identity) [26] using residues 136-183 as a template, as this part has been lost in hydrolases. Figure 3 shows a structure-based multiple sequence alignment of nitrilase from Aspergillus niger with selected templates and sequences of previously published homology models of nitrilases from R. rhodochrous J1 [12, 20] (identity 38%) and Pseudomonas fluorescens [1] (26%). Secondary structure is given as assigned by Procheck [27]. Figure 4A shows a view of the enzyme with the catalytic domain on the left and the active site in the domain center. The three long loops at the entrance of the active site are interesting features of the modelled structure. Loops including the residues that correspond to 236-252 and 55-64 between β2 and α2 and 236-252 between β10-β12 (coloured magenta in Figure 4A) in the primary sequence were found in just one template structure, 2VHH, but these residues were not resolved in the crystal structure [24]. A loop corresponding to 196-207 between β8 and α6 (yellow in Figure 4A) was not found in any template structure (Additional file 4). The nitrilase from Rhodococcus rhodochrous J1 [12] presents similar residues at the corresponding primary sequence positions, and similar external loops in its homology model. The nitrilase from Pseudomonas fluorescens lacks the insertion at the position similar to 196-207 but it has one additional loop between β14 and α7.
Multiple sequence alignment. (A) Multiple sequence alignment of A. niger K10 with template structures 1EMS [21], 1J31 [22], 1F89 [23], 2VHH [24] and 2W1V [25] (letters in upper case) and sequences of homologous nitrilases (letters in lower case) from Rhodococcus rhodochrous J1 (RrJ1) [12] and from Pseudomonas fluorescens (Pf-5) [1]. Clustal W scheme is used for marking similar residues. Amino acids from catalytic triad are strongly conserved; they are marked by red arrow and enclosed in blue rectangles. Secondary structures for template 1EMS and A. niger K 10 nitrilase model are shown above and under the aligned sequences, respectively, with numeration as in [12]. (B) Sequence alignment of A. niger K 10 with 2KIN [26] was used for modelling the C-terminal part. Secondary structure as assigned by Procheck [27] is shown for A. niger K 10 and for 2KIN above and under the aligned sequences, respectively.
The search for homologous structures was done with BLAST [43, 44]. Structures with the highest identity were extracted from the Protein Data Bank and used as templates for modelling. A structure-based multiple sequence alignment was done with the T-Coffee server [53] and manually corrected on the basis of consensus secondary structure prediction [54]. Three-dimensional models consisting of all non-hydrogen atoms were constructed with the package Modeller 9.1 [55] and validated with ProSA [56].
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TWRP 3.4.0-0 has been released officially. This latest update of the recovery adds better support for legacy devices running Android 10, System-as-Root, a new template for TWRP Installer ZIP files, allows flashing of OZIP firmware on Relme/Oppo devices, and also fixes the dreaded sensors bug caused by TWRP.
Installing TWRP is quite an easy task. But before you begin with the instructions, it is required to enable OEM Unlocking and unlock the bootloader of your Android device. That is because without an unlocked bootloader, flashing anything to your device, including TWRP would not be possible.
Now that you have the latest TWRP installed on your Android device, you can use it to flash custom ROMs like LineageOS or Paranoid Android. You can also install mods like Dolby Atmos Port or root your phone by flashing the Magisk zip.
Given the configuration requirements, the above code illustrates acorrectly-configured VolumeSnapshotClass for K10. If theVolumeSnapshotClass does not match the above template, pleasefollow the below instructions to modify it. If the existingVolumeSnapshotClass cannot be modified, a new one can be createdwith the required annotation.
The Silencer removes muzzle flash, makes the Vector much quieter when shooting, and prevents the Vector from making the user appear on radar when firing, at the cost of range. The Vector is a great weapon to use silenced, as it has great accuracy, a large rate of fire, and low damage differential, all of which softens the range penalty.
The Suppressor reduces muzzle flash, makes the Vector K10 quieter when firing, and prevents the Vector K10 from revealing the user on radar when firing, but the Vector K10's four and five shot kill ranges are made 30% shorter. Already starved for range, the Vector K10 isn't as reliant on its damage as other SMGs, so the Suppressor is not the worst fit on the weapon, but some may find the reduction of range on a weapon already lacking in range to be an unwise choice.
The Quickdraw Handle speeds up the aim down sight time to 100 milliseconds, allowing the user to snap onto target in a flash. The attachment has no penalty and a very relevant upside, but its utility, much like with the Laser Sight, will boil down to the user's playstyle.
Some of the barrel attachments allow the user to take full advantage of the Vector CRB's long range. The Muzzle Brake enhances the Vector CRB's three shot kill and four shot kill ranges by 20%, making its main selling card of a long three shot kill range all the sweeter. The Silencer will reduce the Vector CRB's range in exchange for stealth benefits, although the Vector CRB with a Silencer will still retain a longer three shot kill range by default compared to all vanilla SMGs, even besting the K7 in this regard. The Flash Suppressor provides less muzzle flash while not reducing range.
Click a brand to switch the keyboard: Click a game icon to switch demo video: $(document).ready(function () { $(\"img.buttonkeyrazer\").click(function () { var logitech = $(\"div.logikey\"); var razer = $(\"div.razrkey\"); if (razer.css(\"display\") != \"block\") { razer.fadeIn('slow'); logitech.fadeOut('slow'); } }); $(\"img.buttonkeylogitech\").click(function () { var logitech = $(\"div.logikey\"); var razer = $(\"div.razrkey\"); if (logitech.css(\"display\") != \"block\") { logitech.fadeIn('slow'); razer.fadeOut('slow'); } }); }); document.addEventListener(\"DOMContentLoaded\", function () { var videos = { a: Popcorn(\"#a\"), b: Popcorn(\"#b\"), c: Popcorn(\"#c\"), }, scrub = $(\"#scrub\"), loadCount = 0, events = \"play pause timeupdate seeking\".split(/\\s+/g); // iterate both media sources Popcorn.forEach(videos, function (media, type) { // when each is ready... media.on(\"canplayall\", function () { // trigger a custom \"sync\" event this.emit(\"sync\"); // set the max value of the \"scrubber\" scrub.attr(\"max\", this.duration()); // Listen for the custom sync event... }).on(\"sync\", function () { // Once both items are loaded, sync events if (++loadCount == 3) { // Iterate all events and trigger them on the video B // whenever they occur on the video A events.forEach(function (event) { videos.a.on(event, function () { // Avoid overkill events, trigger timeupdate manually if (event === \"timeupdate\") { if (!this.media.paused) { return; } videos.b.emit(\"timeupdate\"); videos.c.emit(\"timeupdate\"); // update scrubber scrub.val(this.currentTime()); return; } if (event === \"seeking\") { videos.b.currentTime(this.currentTime()); videos.c.currentTime(this.currentTime()); } if (event === \"play\" event === \"pause\") { videos.b[event](); videos.c[event](); } }); }); } }); }); scrub.bind(\"change\", function () { var val = this.value; videos.a.currentTime(val); videos.b.currentTime(val); videos.c.currentTime(val); }); // With requestAnimationFrame, we can ensure that as // frequently as the browser would allow, // the video is resync'ed. function sync() { if (videos.b.media.readyState === 4) { videos.b.currentTime( videos.a.currentTime() ); } if (videos.c.media.readyState === 4) { videos.c.currentTime( videos.a.currentTime() ); } requestAnimationFrame(sync); } sync(); }, false); (function () { var gameplayVid = document.getElementById(\"a\"); var logiVid = document.getElementById(\"b\"); var razerVid = document.getElementById(\"c\"); var currentSelection = \"\"; [].forEach.call(document.getElementsByClassName('buttonkeygameicon'), function (btn) { btn.addEventListener('click', function (e) { switch (e.target.id) { case \"overwatch_btn\": if (currentSelection != \"overwatch_btn\") { gameplayVid.src = \"videos/overwatch_demo_gameplay.mp4\"; logiVid.src = \"videos/overwatch_demo_logitech.mp4\"; razerVid.src = \"videos/overwatch_demo_razer.mp4\"; currentSelection = \"overwatch_btn\"; gameplayVid.play(); } break; case \"rocketleague_btn\": if (currentSelection != \"rocketleague_btn\") { gameplayVid.src = \"videos/Aurora_RocketLeague_website_gameplay_cut_2.mp4\"; logiVid.src = \"videos/Aurora_RocketLeague_website_logitech_cut_2.mp4\"; razerVid.src = \"videos/Aurora_RocketLeague_website_razer_cut_3.mp4\"; currentSelection = \"rocketleague_btn\"; gameplayVid.play(); } break; case \"gta5_btn\": if (currentSelection != \"gta5_btn\") { gameplayVid.src = \"videos/Aurora_GTA5_website_gameplay_cut.mp4\"; logiVid.src = \"videos/Aurora_GTA5_website_logitech_cut.mp4\"; razerVid.src = \"videos/Aurora_GTA5_website_razer_cut.mp4\"; currentSelection = \"gta5_btn\"; gameplayVid.play(); } break; case \"dota2_btn\": if (currentSelection != \"dota2_btn\") { gameplayVid.src = \"videos/Aurora_Dota2_website_gameplay_cut.mp4\"; logiVid.src = \"videos/Aurora_Dota2_website_logitech_cut.mp4\"; razerVid.src = \"videos/Aurora_Dota2_website_razer_cut.mp4\"; currentSelection = \"dota2_btn\"; gameplayVid.play(); } break; case \"dodgeball_btn\": if (currentSelection != \"dodgeball_btn\") { gameplayVid.src = \"videos/dodgeball_demo_gameplay.mp4\"; logiVid.src = \"videos/dodgeball_demo_logitech.mp4\"; razerVid.src = \"videos/dodgeball_demo_razer.mp4\"; currentSelection = \"dodgeball_btn\"; gameplayVid.play(); } break; default: console.log(e.target.id); } }) }) }()) 153554b96e
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